Gene-specific epitranscriptomic regulation of alternative splicing and mRNA accumulation. A) Correlation between DTU and m6A changes at selected genes. DTU heatmap displays log₂-fold changes in isoform expression; DRS heatmap shows fold changes in m6A percentage across transcript region of each isoform (NA, no data available). B) Example of transcript-specific m6A changes for differentially spliced At5G25280.1 and At5G25280.2 isoforms in Hpa-treated hakai-1 vs Mock-treated Wt. ONT-DRS tracks display m6A percentages at identified m6A sites (bars). C) Correlation between transcriptional changes and m6A changes of 41 candidate genes. RNA-seq heatmap displays log₂-fold changes in gene expression; ONT-DRS heatmap shows fold changes in m6A percentage averaged across each transcript. D) Browser view of representative candidates MAPK3 and ERD6 (with corresponding heatmap cells indicated in Fig. 1C). RNA-seq tracks display normalized read coverages; ONT-DRS tracks display m6A percentages at identified m6A sites (red bars). C, Col-0; h, hakai-1; M, Mock treatment; H, Hpa treatment.