Response of T cell subsets before and after cryopreservation. Human PBMCs were incubated with CD3/CD28 agonist TransAct. Non-specific binding was compared between immune cell subsets at fresh and day 70 (A). Anti-puromycin GeoMFI for T cell subsets in unactivated and activated conditions was compared at fresh (B). Comparing unactivated (UA-Co) and activated (P-Co) samples, increase in protein translation upon activation is shown for CD4⁺ Naïve (C) and TCM (D) T cells, and CD8⁺ Naïve (E) and TEM (F) T cells. (*P < .05, **P < .01, Friedman Test with Dunn’s multiple comparisons test comparing Days 15, 35 and 70 to fresh only, n=5. Data presented in (B) are descriptive only).