Negative correlation between the m5C rate and C amount across tissues. The m5C rate is the number of m5Cs divided by the total number of transcribed Cs in a supergene. a) The m5C rate of a particular supergene in a tissue decreases with the total C amount of the genes belonging to the supergene in the tissue. The virtually superimposed black and red lines are the linear least-squares regressions for the original and downsampled data, respectively. b) Distribution of Spearman's correlation coefficient between m5C rate and the total C amount across tissues for all supergenes. In each boxplot, the lower and upper edges of a box represent Q1 and Q3 quartiles, respectively; the horizontal line inside the box indicates the MD; and the whiskers extend to the most extreme values inside inner fences, MD ± 1.5(Q3 − Q1). Below each boxplot is the fraction of supergenes showing a negative correlation; all fractions significantly exceed 50% (P < 0.05, binomial test).
