FSP27 deficiency in adipocytes reduces macrophage infiltration in AAA lesions. (a) Schematic illustration of experimental design. Fsp27^fl/fl and Fsp27^AKO mice were fed with a chow diet or an HFD for three months and administered with Ang Ⅱ for four weeks. Aortas were subjected to RNA-seq analysis (n = 4). (b) PCA score plot of the aortic transcriptome data obtained from mice as described in (a). (c) Volcano plot of DEGs using the same data as in (a) (blue, downregulated; red, upregulated; gray, not significant; adjusted P value < 0.05 and absolute fold change (FC) > 2). (d−f) Relative mRNA expression of Mmp2, Mmp9, and Mmp12 in the aneurysmal tissues of mice fed with a chow diet or an HFD and then infused with or without Ang Ⅱ (n = 5). (g−i) Relative mRNA expression of Mmp2, Mmp9, and Mmp12 in the aortas of Fsp27^fl/fl and Fsp27^AKO mice fed with a chow diet or an HFD and then infused with Ang Ⅱ (n = 5). (j) Immunohistochemical staining of the abdominal aortic sections for MMP12 in Fsp27^fl/fl and Fsp27^AKO mice as described in (a). Scale bar, 50 μm. (k) Statistical analysis of MMP12 expression in (j). (l and m) GSEA enrichment plot (l) and heatmap (m) of macrophage migration-associated gene set from the same data as in (a). (n) Relative mRNA expression of F4/80 in the aneurysmal tissues of Fsp27^fl/fl and Fsp27^AKO mice (n = 5). (o) Immunohistochemical staining of the abdominal aortic sections for F4/80 in Fsp27^fl/fl and Fsp27^AKO mice as described in (a). Scale bars, 50 μm. (p) Statistical analysis of F4/80 expression in (o). (q) Representative immunohistochemical staining in serial sections showing the expression of F4/80 and MMP12 in the aortas of Fsp27^fl/fl mice treated with HFD and Ang Ⅱ. Data are presented as the means ± SEM. P values were calculated by Fisher’s exact test (c), two-way ANOVA with Bonferroni test (d−i), and Student’s t-test (m). ns, not significant.