PVAT fat mass and inflammation are reduced in Fsp27^−/− mice. (a and b) Relative mRNA (a) and protein (b) levels of FSP27 in PVAT of WT and Fsp27^−/− mice fed with a chow diet or an HFD for three months were determined by qPCR (a) and western blot analysis (b). (c and d) Body weight (c) or weight of abdominal adipose tissues (d) from WT and Fsp27^−/− mice fed with a chow diet or an HFD for three months (n = 6). (e) Hematoxylin and eosin (H&E) staining of abdominal adipose tissues dissected from WT and Fsp27^−/−mice fed with a chow diet or an HFD for three months. Scale bar, 50 μm. (f) Relative mRNA expression levels of inflammatory genes in PVAT from WT and Fsp27^−/− mice fed with an HFD for three months (n = 5). Data are presented as the means ± SEM. P values were calculated by two-way ANOVA with Bonferroni test (a, c, and d) and two-tailed unpaired Student’s t-test (f).