Anti-OLF1 and anti-OLF2 degrade mutant myocilin through an apparent lysosomal pathway. A) Western blot analysis of the detergent-insoluble fraction from induced iHEK^I477N cells treated with DMSO, the proteasomal inhibitor MG-132, or the lysosomal inhibitor Baf A1 for 24 h following transient transfection of anti-OLF1 (left) or anti-OLF2 (middle). Quantification of Western blots of insoluble myocilin^I477N from two independent experiments, with SD compared to myocilin level in the absence of antibody within each treatment. *P < 0.05; ***P < 0.0001 relative density compared to myocilin^I477N alone. B) Dot blot analysis of spent media fraction shows extremely low levels of secreted myocilin^I477N across these experiments.