Epigenomic modification patterns before and after Poly(I:C) induction

A. Heatmaps showing the distribution of nine ChromHMM chromatin states in CON (left) and TRT (right) cells separately assigned by four histone modifications (H3K27ac, H3K27me3, H3K4me3, and H3K4me1), CTCF-binding sites (CTCF), RNAPII-binding sites (RNAPII), and RNA transcription profiles (RNA). B. Distribution of chromatin state regions in CON and TRT cells. C. Venn diagrams showing the overlap of SEs (left) and super RNAPII-binding regions (right) between CON and TRT cells. D. UP genes correlated with SEs and super RNAPII-binding regions on Chr9. The DEGs are denoted as solid circles over the chromosomal ideogram (yellow for UP and blue for DN). The density curve of CECT-binding sites is shown inside the ideogram, and the peak locations of SEs and super RNAPII-binding regions were shown as bars above the ideogram. E. Example showing an inducible SE (located in Chr9:37,800,000–38,400,000) related with the activation of corresponding chromatin states and up-regulation of the neighbor gene (ZC3H12C). F. Co-localization of UP genes and super RNAPII-binding regions along chromosomes. RNAPII, RNA polymerase II; SE, super enhancer; GT, gene transcription; PB, RNAPII binding; PT, RNAPII transcription; SP, strong promoter; WP, weak promoter; Enh, enhancer; Ins, insulator; Res, repressor; Het, heterochromatin.