MiR-5195-3p regulates ECM metabolism in the vaginal wall tissue of POP patients through the LOX/TGF-β1 signaling pathway. Note: (A) The expression of proteins related to the TGF-β1 signaling pathway in fibroblasts was analyzed using Western blot. Statistical analysis revealed a significant difference (P < 0.05) compared to the POP + inhibitor NC group. A significant difference (^#P < 0.05) was observed compared to the POP + miR-inhibitor + si-NC group. (B) The expression of proteins related to ECM metabolism in fibroblasts was analyzed using Western blot. (C) Immunofluorescence analysis was conducted to examine the expression of proteins related to ECM metabolism in fibroblasts. Statistical analysis indicated a significant difference (P < 0.05) compared to the POP + inhibitor NC group. Furthermore, a significant difference (^#P < 0.05) was observed compared to the POP + miR-inhibitor + si-NC group. A significant difference (&P < 0.05) was also observed compared to the POP + miR-inhibitor + si-LOX + vector group.