Expression of miR-5195-3p and ECM-related proteins in vaginal wall tissues of patients with pelvic organ prolapse. Note: (A) Real-time reverse transcription PCR (qRT-PCR) detection of miR-5195-3p expression in vaginal wall tissues from both non-POP and POP patients. (B) FISH examination of miR-5195-3p expression in vaginal wall tissues from both non-POP and POP patients; (C) immunohistochemistry assessment of ECM metabolism–related protein expression in vaginal wall tissues of POP patients; (D) Western blot analysis of ECM metabolism–related protein expression in vaginal wall tissues of POP patients; (E) Pearson correlation analysis between miR-5195-3p and COL-1 mRNA expression in vaginal wall tissues; (F) Pearson correlation analysis between miR-5195-3p and MMP1 mRNA expression in vaginal wall tissues; (G) Pearson correlation analysis between miR-5195-3p and TIMP1 mRNA expression in vaginal wall tissues; and (H) Pearson correlation analysis between miR-5195-3p and ECM2 mRNA expression in vaginal wall tissues. (^* indicates statistical significance compared to the control group, P < 0.05; ^# indicates statistical significance compared to the POP I-II group, P < 0.05)