Process of performing segmentation from myotube (Myh1) and nuclei (DAPI) fluorescence images. (A) is the original fluorescence image. (B) and (C) are enlarged images of the box from image (A). (D) and (E) are images in which the box is enlarged from the (F) image generated by performing the (G) process. (G) is the process of generating the segmentation image (F) to calculate quantitative parameters of myotube through deep learning and post-processing from the original fluorescence image.