Evaluation of MitoSort using cell hashing data

A. Percentage of barcodes shared between hashtag-based (rows) and MitoSort-based (columns) assignments in multiplexed ASAP-seq data consisting of four donors. B. Heatmap showing the allele frequency of sample-specific mitochondrial germline variants (rows) across singlets (columns) in multiplexed ASAP-seq data. The top legend shows the donor assignments of MitoSort and the hashtag-based approach. C. t-SNE plots of cell barcodes by the frequency of sample-specific mitochondrial germline variants, colored by singlet/doublet classification (left) or by average frequency for the donor-specific mitochondrial germline variants (four right panels). D. Distributions of sample-specific germline variant frequency for each donor across four singlet groups assigned by MitoSort. E. Distributions of counts for each hashtag across the four hashtag-assigned groups. F. Heatmap showing the allele frequency of sample-specific mitochondrial germline variants (rows) across cells (columns) for concordant and discordant doublets. G. Distribution of the number of ATAC fragments per cell in four groups with concordant or discordant assignments. P values were calculated using the Wilcoxon rank-sum test. VAF, variant allele frequency.