Overview of the developed pipeline to identify S-RNase sequences. We performed individually barcoded multiplexed RNA-seq of styles using the Nanopore MinION sequencer and searched for S-RNase–like sequences using previously reported S-RNase sequences as queries. Then, two heterozygous S-RNase sequences were separated by clustering analysis based on sequence similarity, and consensus sequences were generated for each S-allele. Finally, we generated a phylogenetic tree and assigned S-alleles based on the monophyly.