Impact of adolescent and adult stress on the number of parvalbumin (PV)+ and perineuronal nets (PNN)+ cells, and PV+/PNN+ colocalization in the prefrontal cortex (PFC). In male mice, adolescent stress (A) reduced the number of PV+, (B) PNN+, and (C) PV+/PNN+ colocalization in the PFC. Adolescent stress in female mice did not change (D) PV and (E) PNN markers. (F) Female mice stressed during adolescence showed decreased PV+/PNN+ colocalization in the PFC, as indicated by the Menders overlap coefficient. Representative images illustrating PV and PNN expression and their colocalization are placed on the left side of each graph; n = 7–9/group. Data are shown as mean ± SEM. *P < .05; 2-way ANOVA followed by Tukey post-test.