A divergent RNA virus associated with human-infecting P. knowlesi is a member of the unclassified group ‘ormycovirus’. (a) Sequencing coverage of the RdRp of a P. knowlesi-associated viral contig. Trimmed reads were mapped to the assembled contig using BBMap (Bushnell et al. 2017) and visualized with Geneious Prime v2024.0.7. (b) The predicted structure of the putative hypothetical protein of Selindung RNA virus 1. (c) MAFFT alignment of motif C in the palm domain of the P. knowlesi- and Cystoisospora-associated viruses and representative ormycoviruses. (d) Phylogenetic inference of the ormycoviruses aligned with MAFFT. The positions of Erysiphe-associated viruses are denoted with black icons (source: phylopic.org). Red tip dots indicate viruses identified in this study. Tips with names in quotes were previously identified but not named (Forgia et al. 2022). Their corresponding NCBI or “RNA Viruses in Metatranscriptomes database” (RVMT) accession is shown in parentheses. The catalytic triad encoded in each palm domain is denoted in grey. Support values are shown at select nodes as sh-aLRT/UFBoot. Tree branches are scaled to amino acid substitutions.