Phenotype of ProPhas:amiLPAT1 knockdown lines in mature seeds. (A) Seed morphology of the wild type (WT), and ProPhas:amiLPAT1-3 (#15 and #24) and ProPhas:amiLPAT1-4 (#9 and #13) transgenic plants. Bar, 200 µm. (B, C) Measurement of seed weight (B) and size (C) in ProPhas:amiLPAT1 knockdown lines. Data are the mean ±SD from six biological replicates measured from at least 100 seeds for each replicate. (D, E) Absolute amount per dry weight (D) and fatty acid composition (E) of triacylglycerol (TAG) in ProPhas:amiLPAT1 knockdown lines. The asterisks indicate statistical significance compared with the WT by one-way ANOVA (B–D) and two-way ANOVA test (E) (*P<0.05, **P<0.01, ***P<0.001).