Fig. 3.
Phenotype analysis of transgenic plants ProPhas:amiLPAT1 WT. (A) An overview (upper panels) and magnified view (lower panels) of mature siliques of the wild type (WT), ProPhas:amiLPAT1-3 WT (lines #15 and #24), and ProPhas:amiLPAT1-4 WT (lines #9 and #13). Shrunken seeds are marked with a black asterisk. Bars, 1 mm. (B) Length of siliques and percentage of aborted seeds shown in (A). Data are the mean ±SD from 10 siliques in each genotype (n=10). (C) RT–qPCR analysis of the relative transcript level of LPAT1 in rosette leave, young siliques, and mature siliques of the WT and ProPhas:amiLPAT1 knockdown lines. Data are the mean ±SD from three biological replicates, each with a median of three technical replicates. The asterisks indicate statistical significance compared with the WT by one-way ANOVA test (*P<0.05, **P<0.01, ***P<0.001).

Phenotype analysis of transgenic plants ProPhas:amiLPAT1 WT. (A) An overview (upper panels) and magnified view (lower panels) of mature siliques of the wild type (WT), ProPhas:amiLPAT1-3 WT (lines #15 and #24), and ProPhas:amiLPAT1-4 WT (lines #9 and #13). Shrunken seeds are marked with a black asterisk. Bars, 1 mm. (B) Length of siliques and percentage of aborted seeds shown in (A). Data are the mean ±SD from 10 siliques in each genotype (n=10). (C) RT–qPCR analysis of the relative transcript level of LPAT1 in rosette leave, young siliques, and mature siliques of the WT and ProPhas:amiLPAT1 knockdown lines. Data are the mean ±SD from three biological replicates, each with a median of three technical replicates. The asterisks indicate statistical significance compared with the WT by one-way ANOVA test (*P<0.05, **P<0.01, ***P<0.001).

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