Characterization of cell types associated with secondary follicles. (A) Immunofluorescence for DDX4 (green), AMH (yellow), and KRT19 (magenta) in secondary follicles present in cryo-thawed cOVA, cryo-thawed tOVA, and freshly isolated tOVA before (D0) and after culture. Dashed line highlights secondary follicles. Scale bar is 50 µm. (B) Immunofluorescence for ACTA2 (green) and CD68 (magenta) in the stromal tissue surrounding secondary follicles present in cryo-thawed cOVA, cryo-thawed tOVA, and freshly-isolated tOVA before (D0) and after culture. Dashed line highlights secondary follicles. Scale bar is 50 µm. Mc_D8, culture after McLaughlin et al. (2018) for 8 days; Xu_W1/W3, culture after Xu et al. (2021) for 1/3 weeks.