Effect of CB1 stimulation on kinase activity and p53 nuclear translocation. (A) Proliferating male and female Apoe^−/− BMDMs treated with vehicle, 10 µM AM281 or 1 µM ACEA for 24 h (n = 4). (B) Top 10 enriched kinase pathways and (C) heatmap of kinase activity in male Apoe^−/− BMDM treated with vehicle or 1 µM ACEA for 60 min (n = 4). Values indicate mean kinase statistic; significantly different kinases with median final score > 1.2 are shown. (D) Interaction map showing kinase pathways regulated in ACEA-stimulated male BMDMs. (E) Proliferation rates in male BMDM treated with vehicle, AM281 (10 µM) and p53 inhibitor (PFT-α; 25 µM) for 24 h (n = 3). (F and G) Phospho-p53 immunostaining (green) and quantification of nuclear p53 translocation in male Apoe^−/− BMDMs treated with vehicle, 1 µM ACEA or 10 µM AM281 for 60 min. Nuclei were stained with Hoechst33342 (blue); scale bar: 50 μm (n = 4). Each dot represents one mouse and all data are expressed as mean ± S.E.M. One-way ANOVA followed by Tukey test (H and I), two-way ANOVA followed by Tukey test (A, E, and G). Male and female were analysed independently (A).