Strong correlation between cell-type-specific HMGs and HEGs. (A) Identification of HM loci from the difference (green curve) between the mobility profile obtained with the GNM (for IMR90 chromosome 17; yellow curve), and that averaged across 16 cell lines (blue curve); HM loci are defined as those exhibiting the top 10% mobility, shown by red dots. Genes located in HM loci, HMGs, are shown by green dots. (B) The procedure in (A) is repeated for all chromosomes; the resulting list of HMGs for IMR90 chromatin is used for screening against the cell-type-specific HEGs compiled in the ARCHS⁴ database. The similarities between the HMGs of the query cell type and the HEGs of the 125 cell types in ARCHS⁴ are quantified by the Jaccard index, and rank-ordered for each query cell type. The bar plot illustrates how IMR90 yields the highest Jaccard index, demonstrating that knowledge of HMGs is sufficient to distinguish cell identity due to strong correlation to the cell-specific HEG profile. (C) The procedure repeated for different cell types shows that the queried cell type is extracted as top-ranking cell type (from the pool of 125 in ARCHS⁴) based on high similarity of its HMGs to its HEGs. See more details in Zhang et al. [51].