A, Number of CSF samples analyzed each year in Botswana, 2016–2022, with the proportion receiving TB-specific investigation represented in black. Samples from Princess Marina Hospital, Gaborone, were excluded as universal Xpert MTB/RIF Ultra was implemented since 2020. B, Yearly variation of the proportion of TB-specific investigations performed on CSF each year excluding Princess Marina Hospital, Gaborone, 2016–2022. Abbreviations: CSF, cerebrospinal fluid; TB, tuberculosis.