Graphical representation of representative alleles and their corresponding nomenclature. a) Alleles present in the ura4 locus and their corresponding names. In order: (1) The wild-type locus; (2) A deletion of the ORF without replacement (e.g. scar-free CRISPR-Cas9); (3) A deletion allele in which the ura4 locus was replaced by a KanMX6 cassette. Note that the deletion allele ura4Δ is used both to indicate the locus of insertion and the fact that the ura4 ORF is missing; (4) This allele name can represent the insertion of P.act1-GFP-ase1 at either side of the ura4 locus since the transcription orientation is not captured in the allele name; (5) For the same reason, a construct containing 2 flanking ura4 alleles can have 2 synonymous names when inserted in the ura4 locus; (6) Allele names should capture the insertion locus and the relative position of features, rather than how alleles were generated or their genetic meaning. Therefore, in a case where an allele of the insertion locus (ura4+ allele in the image) is in the middle of the construct, we use ura4Δ:: to indicate the insertion locus, followed by the construct name, including the ura4+ allele. b) Special cases in which an allele name contains 2 gene symbols (ase1 and ura4), and one of them is also the locus of insertion. The wild type locus is shown on top, and the resulting alleles and their names at the bottom: (left) If inserted in the ura4 locus replacing the ura4 ORF, the allele name starts with “ura4Δ::”; (center) If inserted in the ura4 locus keeping the ura4 ORF, the allele name starts with “ura4+::”; (right) If inserted in the ase1 locus, the allele name starts with “ase1-GFP::”.