Sustained Ang II up-regulated cardiac and vascular structural, inflammatory, and calcium regulatory proteins and down-regulated mitochondrial proteins. Proteomic workflow (A) in both mouse tissues and human cell line, with corresponding results illustrated in (B–I). Volcano plots (with corresponding Venn diagram in the inset) displaying proteins identified in our study in mice (B and G) and human cell lines (D and I) with structural (red), calcium regulatory (orange), inflammatory (brown), and mitochondrial (blue) proteins. Up-regulated (yellow) and down-regulated (purple) biological processes (top lollipop panel) and cellular components (bottom lollipop panel) in TA (C) and LV (H). Venn diagrams display commonly identified up- and down-regulated proteins from our study and published human proteomic data set (E–K). Venn diagram comparing the TA mouse proteome from this study with the TA proteome of human aortic remodelling data set from Herrington et al.¹⁸ (E). Venn diagram compared the HASMC proteome from this study with the human aortic remodelling TA proteome (F). Venn diagram compared the LV mouse proteome from this study with the LV proteome of human hypertensive data set from Coats et al.¹⁹ (J). Venn diagram compared the HCF proteome from this study with the human hypertensive LV proteome (K). GO, gene annotation; BP, biological process; CC, cellular component; HTN, hypertensive; NT, normotensive; Veh, vehicle; Ang II, angiotensin II; Ctrl, control; Sal + Veh, vehicle-treated normotensive mice; AngII + Veh, vehicle-treated hypertensive mice; HCFs, human cardiac fibroblasts; HASMCs, human aortic smooth muscle cells.