Genomic characterization and phylogenetic analysis of CFV/JKCDC22 (in red), a novel orthomyxovirus. (a) Genomic structures of the five CFV/JKCDC22 segments and comparison of the five conserved RdRp motifs. (b–f). The ML phylogenies of the five CFV/JKCDC22 segments. All representative species within the family Orthomyxoviridae and other unclassified anuran-related orthomyxoviruses were included in the analyses, with the members within the genera Thogotovirus and Quaranjavirus being collapsed. Numbers next to nodes are SH-aLRT support (%)/ultrafast bootstrap support (%). Abbreviations: AIV_in, Alphainfluenzavirus influenzae; BIV_in, Betainfluenzavirus influenzae; DIV_in, Deltainfluenzavirus influenzae; GIV_in, Gammainfluenzavirus influenzae; ISV_sa, Isavirus salaris; MKV_tr, Mykissvirus tructae; QJV, Quaranjavirus; SDV_pi, Sardinovirus pilchardi; Thogoto, Thogotovirus; Quaranja: Quaranjavirus; CTIV, Cane toad influenza-like virus; OCFIV, Ornate chorus frog influenza-like virus; WATIV, Wuhan asiatic toad influenza virus; CFV, Chadfomvirus; FILV, Flounder influenza-like virus; SILV, Sturgeon influenza-like virus; LSILV, Long-snouted seahorse influenza-like virus; GCILV, Grass carp influenza-like virus.