Three-way categorization of top differentially expressed genes (P < .05) of “Ca. Methanoperedens” between 0% and 1.5% salinities. Categories include central metabolism (A), morphological change and locomotion (B), and overall stress-related response (C). Up- and downregulated transcripts are indicated in the y-axis for (A) and (B) and x-axis for (C). Up- and downregulated genes are referred to as log₂ fold change. For (B) and (C), additional annotation includes a plasmid-harboring and potential phage-related transcript, FlaI_TIVSS and ltrA, respectively.