Fig. 8
Phenotypic observations and reporter assays of 11700RepYFP in the mir390/11700ge mutants and MIR390/11700OE plants. (A) Secondary structure prediction of the wild type and edited miR11700 precursors. The mature sequence is highlighted in red and the star sequence is highlighted in blue. The mir390ge mutant was used as the genetic background to mutate miR11700. Northern blotting of miR390 and miR11700 in the mir390/11700ge mutants (B) and MIR390/11700OE plants (C), respectively. (D) Vegetative growth of the 18-day-old mir390/11700ge mutants. Scale bars, 1 cm. (E) qRT-PCR analysis of MpCYP78A101 in the gemmae of the mir390/11700ge mutants. (F) Vegetative growth of the 18-day-old MIR390/11700OE plants. Scale bars, 1 cm. (G) qRT-PCR analysis of MpCYP78A101 (i) and MpARF2 (ii) in the thallus of MIR390/11700OE plants. (H) YFP-transient reporter assays of 11700RepYFP in the mir390/11700ge mutants and MIR390/11700OE plants (i). The YFP intensity was quantified using more than 20 images captured by a fluorescence microscope (ii). Tak-1 was used as a control. Error bars represent the standard error from three biological replicates. (I) Relative cell area ratios of various plants in response to Tak-1 plants without NAA treatment. Epidermal cells in the central region of gemma were used for area calculation.

Phenotypic observations and reporter assays of 11700RepYFP in the mir390/11700ge mutants and MIR390/11700OE plants. (A) Secondary structure prediction of the wild type and edited miR11700 precursors. The mature sequence is highlighted in red and the star sequence is highlighted in blue. The mir390ge mutant was used as the genetic background to mutate miR11700. Northern blotting of miR390 and miR11700 in the mir390/11700ge mutants (B) and MIR390/11700OE plants (C), respectively. (D) Vegetative growth of the 18-day-old mir390/11700ge mutants. Scale bars, 1 cm. (E) qRT-PCR analysis of MpCYP78A101 in the gemmae of the mir390/11700ge mutants. (F) Vegetative growth of the 18-day-old MIR390/11700OE plants. Scale bars, 1 cm. (G) qRT-PCR analysis of MpCYP78A101 (i) and MpARF2 (ii) in the thallus of MIR390/11700OE plants. (H) YFP-transient reporter assays of 11700RepYFP in the mir390/11700ge mutants and MIR390/11700OE plants (i). The YFP intensity was quantified using more than 20 images captured by a fluorescence microscope (ii). Tak-1 was used as a control. Error bars represent the standard error from three biological replicates. (I) Relative cell area ratios of various plants in response to Tak-1 plants without NAA treatment. Epidermal cells in the central region of gemma were used for area calculation.

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