Phenotypic observations and reporter assays of 78ARep^YFP in the amiR-TASI78A^OE plants. (A) Vegetative growth of the 18-day-old amiR-TASI78A^OE plant G3 lines. Scale bars, 1 cm. (B) Northern blotting of amiR-tasi78A in amiR-TASI78A^OE plants. U6 served as a loading control. (C) qRT-PCR of MpCYP78A101 in the amiR-TASI78A^OE plants. MpEF1-alpha was used as a reference gene. Error bars represent the standard error from three biological replicates. (D) YFP transient reporter assays of 78ARep^YFP in the amiR-TASI78A^OE plants (i). The quantified relative YFP intensity of the data was calculated from more than 30 images captured by a fluorescence microscope (ii). Tak-1 plants were used as control. Error bars represent the standard error from three biological replicates.