Only subtype B infections are included. (A) Phylogenetic tree comparison of HIV of protease and reverse transcriptase gene sequences from the TTIMS programs and selected LANL reference sequences from 2015–2020. (B) Mean genetic distances comparing TTIMS sequences and LANL references sequences. The violin plot and results of the Kruskal-Wallis (KW) 1-way analysis of variance test show the diversity of the TTIMS HIV sequences are significantly greater than the diversity of available reference sequences from LANL. Reference Genome: GenBank ID K03455.1 Human immunodeficiency virus type 1 (HXB2) complete genome HIV1/HTLV-III/LAV reference genome.