Genome assembly and quality metrics for the Mojave poppy bee genome. A) GenomeScope2 plot of k-mer frequency at varying depth of coverage for the initial assembly B) Blob plot for Mojave poppy bee initial genome assembly. Each blob represents a contig, colored according to the phylum with the best match using BLAST hits to the NCBI nucleotide database or Diamond BLAST hits to the Uniprot protein database. Most blobs are assigned to Arthropoda, with only a few assigned to fungi, plants, or Chordata. C) BlobToolKit snail plot of the final draft genome assembly after removal of non-arthropod contigs. The main plot is divided into 1,000 size-ordered bins around the circumference with each bin representing 0.1% of the 327,936,261 bp assembly. The distribution of sequence lengths is shown in dark gray with the plot radius scaled to the longest sequence present in the assembly (31,838,323 bp, shown in red). Orange and pale-orange arcs show the N50 and N90 sequence lengths (17,524,680 and 3,032,719 bp), respectively. The pale gray spiral shows the cumulative sequence count on a log scale with white scale lines showing successive orders of magnitude. The blue and pale-blue area around the outside of the plot shows the distribution of GC, AT, and N percentages in the same bins as the inner plot. A summary of complete, fragmented, duplicated, and missing BUSCO genes in the hymenoptera_odb10 set is shown in the top right. D) Plot of cumulative sequence length covered by contigs. The gray line shows cumulative length for all contigs, while the blue line shows cumulative length for Arthropoda contigs. In the final draft genome assembly with non-arthropod contigs removed, the blue and gray lines overlap.