Palmitic acid (PA) exposure upregulated ACBP expression and impaired embryonic development. (A) Western blot of ACBP at 24 h after various concentrations of PA treatment in HTR-8/SVneo cells. (B) Quantified protein levels of ACBP, relative to the protein levels of β-actin. (C) HTR-8/SVneo cells were stimulated with various concentrations of PA, and the mRNA level of ACBP, examined 24  h after the treatment. (D) Western blot of ACBP at 6, 12, and 24h post-treatment with 0.5  mM PA. (E) Quantified protein levels of ACBP at 6, 12, and 24h post-treatment with 0.5 mM PA, relative to the protein levels of β-actin. (F) Quantitative analysis for ACBP mRNA. (G) The protein levels of ACBP detected by Western blot in hTSCs-EVT treated with 0.5 mM PA for 24 h. (H) Quantified protein levels of ACBP, relative to the protein levels of β-actin. (I) Quantitative analysis for ACBP mRNA. (J) Placental ACBP protein levels detected using Western blot. (K) Quantified protein levels of ACBP, relative to the protein levels of β-actin. (L) Quantitative analysis for ACBP mRNA. (M) The uterine appearance on D14 (n=10, Scale bar=1 cm). The black arrow indicates embryo resorption with severe uterine bleeding at D14. (N) Resorption rate of D14 in normal and PA-exposed mice (resorption implantation site per mouse/total implantation site per mouse). (O) Fetus weight (P) Placental weight. *P<0.05, **P<0.01, ***P<0.001. Each result is from three independent experiments.