The proportions and types of mutated VviEDR1 increased with the leaves development, resulting in strong powdery mildew resistance in the mature leaves of VviEDR1-chi lines. A) The leaf development process of VviEDR1-chi lines, the stars indicated the morphology of the same leaf at the 0th, 6th, 13th, and 20th day of recording; B) The proportions and types of mutated VviEDR1 changed with the leaves development of VviEDR1-chi lines; C) Representative images showed the trypan blue-stained of young (leaf nos. 5) and mature (leaf nos. 10) leaves of WT and VviEDR1-chi lines, which were infected by the powdery mildew naturally occurred in greenhouse, the yellow arrow points to the normal haustoria of powdery mildew, and the red arrow points to the abnormal haustoria; D) The proportion of normal haustoria and abnormal haustoria in the leaves of WT and VviEDR1-chi lines. Each data point represents the mean ± SD of five biological replicates, asterisks indicate significant differences in comparison with the WT as determined by Student's t-test (*P < 0.05), NA indicate no significant differences (P > 0.05) in comparison with the WT.