Reduction of MEcPP levels and ICS expression in 35S:MTS1 tomato transgenic plants. A) MEcPP content was measured (nmol/g FW, fresh weight) in overexpressing 35S:MTS1 plants and their control MM transgenic plants (n = 4) carrying an empty vector (MM) upon infection with Pst. Mock represents the noninoculated plants. Statistically significant differences (ANOVA, P < 0.05) between genotypes and infected (Pst) or mock-treated plants are represented by different letters. Levels are represented as mean ± Sd of 4 biological replicates. B)ICS expression levels in infected 35S:MTS1 plants and their corresponding parentals with empty vector (MM; n = 3). The y axis represents the value of the Ct increment (ΔΔCt). Values were normalized to Actin gene. Expression levels are represented as mean ± Sd of 3 biological replicates of 1 representative experiment. Statistically significant difference (t test, P < 0.05) between treated and nontreated is represented by an asterisk (*).