a) The immunostaining images of the vinculin (red) expression and the corresponding images merged with Phalloidin (green) and nuclei (blue) of hADSCs cultured on the gradient Lam-111 LB-film for 12 h. Scale bar is 20 µm. b) and c) are the statistical analyses of mean focal adhesion area (FA) area and mean focal adhesion (FA) aspect ratio. At each position, eight images of individual cells were processed. Error bars are the standard error of mean, and significant differences (one-way ANOVA with Bonferroni test, *P << 0.05).