Comparison of compensation methods and uncorrected counts on semi-synthetic data (spillR and spillR-naive are set to impute spillover values with 0). The vertical dashed line helps to interpret the spillover correction. It indicates the original mode of the bead distribution of Yb172Di at 2.7, before overwriting it with the first peak of the real observations of Yb173Di. Counts are arcsinh transformed with cofactor of five (Bendall et al. 2011). The zero percentages are averages over all three experiments