Impact of Tug1 downregulation on its target genes in various tissues of FRDAkd mice. (A) Venn diagram illustrating the cross-comparison of Tug1 targets identified from RNA pull-down assay using Tug1-transfected human glioma cells and other experimental data, with the list of differentially expressed genes uncovered via transcriptomic analysis in the heart, cerebellum and DRG of FRDAkd mice. (B–F) Expression changes in Tug1 targets within (B) heart, (C) blood, (D) muscle, (E) brain and (F) spinal cord tissues of FRDAkd mice treated with dox for 0 and 6 weeks (Fxn knockdown), followed by an 8-week rescue phase (Fxn recovery). These alterations were assessed through qRT-PCR experiments. Four samples were taken at each time point (n = 4). One-way ANOVA was used for statistical analysis. Data are presented as mean ± SEM, with significance denoted as *P ≤ 0.05, **P ≤ 0.01 and ***P ≤ 0.001.