Amino acid alignment of reference Peribunyaviridae RdRp sequences and the novel and highly divergent Mystacina tuberculata metagenome-derived peribunyavirus (bold) showing motifs B–E. The MAFFT alignment and the L-INS-I algorithm were selected to align the sequences in Geneious Prime (Katoh et al. 2002). The flame symbol highlights that the Mystacina tuberculata metagenome-derived peribunyavirus was identified using a protein structural similarity-based approach rather than an amino acid sequence similarity-based approach.