Vitamin E pre-exposure negates plastic flaming glutathione oxidation response. 16HBEs expressing oxidation-sensitive roGFP grown in submerged culture on coverslips were exposed during live-cell imaging to a PBS negative control, 100 mM peroxide (H₂O₂) positive control, or 25 μg/cm² concentrations of flaming PIEs with or without a 3-h preincubation of 20 µM Vitamin E a-tocopherol in PBS. Analyses of real-time roGFP measurements of Oxidized (GSSG) to reduced (GSH) glutathione over time showed that 3-h preincubation of 20 mM Vitamin E a-tocopherol negates the 25-μg/cm² glutathione oxidation response over time (A). Graphing maximum GSSG/GSH oxidation responses of each experiment show a significant decrease in glutathione oxidation between flaming 25 μg/cm² exposures with and without a-tocopherol preincubation (B). Data are represented as means±SEM. Statistical analysis was performed with 1-way ANOVA and Sidak post hoc test comparing PIE exposure groups, significant p-values are shown, n = 3 independent experiments (1 well per experiment) per exposure condition.