Scatter plots showing data from segmented collagen quantification. PSR-stained heart sections from 6–8-week-old animals were segmented in two dimensions [latero-lateral composition (A), strata (B)] and analyzed using the macro. Statistics were performed by two-way ANOVA and Šidák’s multiple comparisons test. n = 9. Abbreviations: EP: epicardium, SEP: subepicardial layer, M: myocardium, SEN: subendocardial layer, EN: endocardium, (r): right ventricle, (s): septum, (l): left ventricle.