Critical cargo loading in the extracellular vesicles (EVs). A, Flowchart illustrating the experimental procedures for the removal of proteins in EVs. B, Expression of GapA and Mycoplasma gallisepticum (MG) load in HD11. C, Relative expression levels of Toll-like receptor (TLR) 2 and interferon (IFN) γ messenger RNA (mRNA) in HD11 cells. D, E, Effect of cargo of EVs on inflammatory factors, displayed as relative expression levels of tumor necrosis factor (TNF) α and interleukin 1β, 10, and 6 (IL-1β, IL-10, and IL-6) mRNA in HD11 cells. Data represent means and standard deviations of ≥4 independent experiments. **P < .01 (vs MG group).