None of the three MpTPCs are required for electrical signal and Ca²⁺ wave propagation. (A) Binarized time lapse imaging of cytosolic Ca²⁺ in WT and Mptpc1-1^ko, Mptpc2-2 tpc3-2^ko expressing GCaMP6f-mCherry after wounding. The magenta arrow indicates the wounded site. Scale bar = 3 mm. (B) Velocity of Ca²⁺ wave in WT, Mptpc1-1^ko and Mptpc2-2 tpc3-2^ko. n = 7–10. mean ± SD. (C) Velocity of electrical signal in WT, Mptpc1-1^ko and Mptpc2-2 tpc3-2^ko. n = 5–35. P-values were calculated by Mann–Whitney test.