Fruit ripening and expression profile of MaCCCH33-like2. (A) Phenotype of ‘Fenjiao’ banana treated by ethylene and 1-MCP. Modifications in physiological indicators throughout the storage period: color index (B), firmness of the fruit (C) and ethylene production (D). (E) The expression level of MaCCCH33-like2 transcript in the pulp during the storage and ripening stages of banana fruit. The transcription of MaCCCH33-like2 at various time points is compared relative to day 0, which is set as 1. Different letters represent statistically significant differences (P < 0.05) in different temperature storage conditions. ETH: ethephon treatment; 1-MCP400: 400 nl L−1 of 1-MCP treatment for 1 h. 1-MCP-treated fruit was stored for 5 d and then treated by ethephon for ripening (5 d + ETH). (F) The subcellular localization of MaCCCH33-like2 in tobacco leaves. The empty-GFP-positive control and MaCCCH33-like2-GFP were temporarily introduced into tobacco leaves, resulting in transient expression. The fluorescence microscope was used to capture images, specifically detecting green fluorescence. The scale bars in the images represent a length of 50 μm.
