PCR amplification and Sanger sequencing validated the high-confidence SVs A. An example of Sanger sequencing validating a deletion event in chromosome 8. B. PCR amplification rates for different genomic regions. The green line represents the amplification success rate. C. Consistency rates of SV type, SV length, and breakpoint position between the Sanger sequenced SVs and the high-confidence SVs. Uncertain sites were excluded.