WGCNA of broccoli transcripts. A Dendrogram of genes based on co-expression network analysis. Each leaf in the tree corresponds to an individual gene. Twenty-nine modules, each associated with a different color, formed from the major tree branches. B Association between modules and GSL content. The color of each module is the same as that in A. The color scale indicates the correlation. Each cell contains the number of corresponding correlations and P values. C Heat maps and bar graphs of co-expressed genes in the MEgreen module. D KEGG pathway analysis of co-expressed genes in the MEred module. E, F Co-expression network and gene expression profile of the MEgreen module from WGCNA. Transcription factors with strong regulatory relationships (weight > 0.3) are indicated by red circles, the GLS biosynthesis genes, glutathione metabolism genes, amino acid biosynthesis genes and cysteine and methionine metabolism genes of the MEgreen module with strong regulatory relationships (weight > 0.3) to TFs are indicated by yellow, purple, orange, and peach circles.