Comparison of CCC patterns and microenvironment patterns in the mouse seminiferous tubule datasets. (A) Microenvironment pattern and CCC pattern. The left is microenvironment pattern of WT3 dataset. The middle is CCC pattern of WT3 dataset based on our method IGAN. The right is CCC pattern of WT3 dataset based on COMMOT. (B) Comparison of the consistency between the CCC pattern and the microenvironment pattern in IGAN and COMMOT methods. The 12 data point represent 6 sending signal CCC pattern and 6 receiving signal pattern. As the value decreases, there is a greater resemblance between the microenvironment pattern and CCC pattern. The Wilcoxon test P = 0.0015. (C) Left: CCC activity overview of WT1 dataset. Right: sperm development stages [22]. (D) CCC of CALM1 and CALM2 in cell-type level. The thickness of the flow represents the strength of the interactions. (E) GO analysis of the high activity ligand in WT3 dataset.