Phylogenetic position and spatiotemporal expression characteristics of ClNAM. (A) Phylogenetic position of ClNAM; the ClNAM (Cl43444) is the sequence used in this study. (B) Protein sequences of NAM are conserved in Chrysanthemum species. (C) Results of subcellular localization of ClNAM. (D–G) Expression patterns of ClNAM in the doming stage (S1), early stage of floret primordia initiation (S6), early stage of corolla primordia formation (S8), and late stage of corolla primordia formation (S10). bar = 200 μm. At: Arabidopsis thaliana; Cl: Chrysanthemum lavandulifolium; Cm: Chrysanthemum makinoi; Cmo: Chrysanthemum × morifolium; Cn: Chrysanthemum nankingense; Cs: Chrysanthemum seticuspe; Han: Helianthus annuus; Mm: Mikania micrantha; Ls: Lactuca sativa.