Interaction of MrMYB cluster members with MrbHLH1. A) Yeast two-hybrid assays revealed the interactions of MrMYB cluster members with MrbHLH1. The DDO (double dropout supplements) medium (SD/-Leu/-Trp) and QDO (quadruple dropout supplements) medium (SD/-Trp/-Leu/-His/-Ade) were used as selective media. AbA, aureobasidin. B) Firefly LCI on the direct interactions of MrMYB cluster members with MrbHLH1 in N. benthamiana leaves. Three independent biological replicates, with each replicate including three leaves taken from one plant, were performed. The experiments were repeated three times independently with similar results.