Interaction of MrMYB cluster members with the promoters of anthocyanin biosynthetic/transport-related genes. A) Effects of MrMYB cluster members, alone or in combination with MrbHLH1, on the promoter activity of anthocyanin biosynthetic/transport-related genes measured by dual-luciferase assays. Averaged data (± SE) from three independent biological replicates, with each replicate including six leaf discs (6 mm in diameter) taken from one plant, are shown. Statistical analysis was performed using one-way ANOVA test and same letter indicates no significant difference (P < 0.05). B) Yeast one-hybrid assays on the interactions of MrMYB cluster members with the promoters of MrCHI, MrANS, and MrGST1. C) EMSA showing the binding of MrMYB1.1n and MrMYB1.3-1 to the promoters of MrCHI, MrANS, and MrGST1.
