Analysis of differential expressed genes between WT and hsfA9 under CDT. (A) Go enrichment analysis of the significantly up- and down-regulated genes between comparisons of WT-CDT and hsfA9-CDT. Dot color represents log10 (-adjust P-value) and dot size represents the number of genes. (B) Heatmap of expression patterns for the top 50 CDT-suppressed genes. Hierarchical clustering analysis was performed for both samples and genes using the Euclidean distance as the similarity metric. (C) RT-qPCR analysis of eight representative genes (HSFA2, HSP17.6, HB1, ERF019, NLM1, PYL4, CIB1 and HSP101) in response to CDT treatment in hsfA2, hsfA9 and hsfA2/hsfA9 mutants. Error bars show SD from three replicates. Letters in the bar plots indicating significant differences (P < 0.05).