Expression patterns of PaFTL in P. aphrodite. (A) PaFTL expression levels in leaves and axillary buds. (B) PaFTL expression patterns following high– (10 h of light at 32°C/14 h of dark at 28°C) or low–ambient temperature (10 h of light at 24°C/14 h of dark at 18°C) treatment of leaves and axillary buds. Samples were harvested at 3-day intervals, and UBIQUITIN10 (UBI10) was used as an internal control. Error bars represent ±SD of three biological replicates. (C) In situ hybridization patterns of PaFTL in axillary buds subjected to high temperature (H0), 7 (L7) and 18 (L18) d of low-temperature treatment. Only FTL expression patterns appeared in H0, while background levels of PaFTL expression were detected in L7 and L18. (D) Relative expression levels of PaFTL in the leaves and axillary buds of juvenile and mature plants cultivated at high ambient temperatures (10 h of light at 32°C/14 h of dark at 28°C). The axillary buds of juvenile plants were harvested from 15 different plants and combined, and the error bars represent ±SD of three technical replicates normalized to UBI10 expression. Other samples were harvested from three different plants, and the error bars represent ±SD of three biological replicates normalized to UBI10 expression. (E) Expression patterns of PaFTL in response to low ambient temperatures (10 h of light at 24°C/14 h of dark at 18°C) in juvenile and mature plants. Plants were harvested at 3-day intervals, with samples collected at ZT8. Error bars represent ±SD of three biological replicates normalized to UBI10 expression. Standard errors were calculated from the means of three biological replicates.