Cardiac miR-222 increases in pathological cardiac hypertrophy and heart failure induced by pressure overload. (A and B) HW/TL (A) and LW/TL (B) ratios of sham or TAC mice at 3, 7, and 14 days after TAC (n = 3–4 in each group). (C–E) A qRT-PCR analysis of hypertrophy markers (C), miR-222 (D), and pri-miR-222 (E) in hearts from sham or TAC mice at different time points. Data are shown as fold induction of gene or miR expression normalized to GAPDH (C), U6 (D), and GAPDH (E), respectively (n = 3–4 hearts in each group). (F and G) HW/TL (F) and LW/TL (G) ratios of mice 42 days after sham and TAC surgery (n = 3–4 hearts in each group). (H) A qRT-PCR analysis of miR-222 in hearts from mice 42 days after sham or TAC. Data are shown as fold induction of gene or miR expression normalized to U6 (n = 3–4 hearts in each group). (I) A qRT-PCR analysis of miR-222 in adult cardiomyocytes and non-cardiomyocytes isolated from mice 2 weeks after sham and TAC surgery. Data shown as fold induction of gene or miRNA expression normalized to U6 (n = 5 in each group). The error bars represent SEM. *P < 0.05 compared with respective controls using Student’s t-test or one-way ANOVA with Tukey’s post hoc test.