Gly357* mutant mice develop a severe ataxia. (A) Representative image of 1-year-old wild-type (WT) mouse showing paw spaying and Gly357* mouse showing paw clasping. (B) Limb clasping analysis in wild-type and Gly357* mice at 3, 4, 5, 6, 9 and 12 months of age (3 months, WT n = 7, Gly357* n = 6; 4 months, WT n = 12, Gly357* n = 9; 5 months, WT n = 10, Gly357* n = 12; 6 months, WT n = 6, Gly357* n = 5; 9 months, WT n = 17, Gly357* n = 13; 12 months, WT n = 8, Gly357* n = 8). (C) Representative image of wild-type and Gly357* mice on the horizontal ladder. The Gly357* mouse displays a paw slip. (D) Quantification of paw slips in wild-type and Gly357* mice at 6, 9 and 12 months of age (6 months, WT n = 8, Gly357* n = 7; 9 months, WT n = 12, Gly357* n = 9; 12 months, WT n = 10, Gly357* n = 11). (E) Representative paw print image of wild-type and Gly357* mice. (F–J) Quantification of toe spreading (F), stride length (G), step length (H), base of support (I) and paw area (J) in WT and Gly357* mice at 6 and 12 months of age (6 months, WT n = 7–8, Gly357* n = 11–14; 12 months, WT n = 9–12, Gly357* n = 13–14). Data are presented as mean ± standard error of the mean (SEM). ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05; one-way ANOVA followed by Sidak’s post hoc test (B and D); one-way ANOVA followed by Tukey’s post hoc test (F–J).